Figure 4.
Interaction of the cytosolic domains of CD63 and mutated CD63 with μ2 and μ3A in the yeast two-hybrid system. Yeast cells were transformed with bait constructs (LexA fusion) containing the C-terminal cytosolic domains of CD63, and the mutant forms AYEVM, GAEVM, GYAVM, GYEVA, GYAVM, GYEAM, GYEVA, and GYEVI and a prey construct (either VP16-μ2, VP16-μ3A). Transformed yeast were grown on agar plates in the absence of histidine for 3 d (μ3A) or 10 d (μ2). All transformed yeast grew in the presence of histidine and no transformed yeast containing “empty” VP16 grew in the absence of histidine.