Figure 8.

Steady-state localization of CD63 and GYEVI constructs in stably transfected mocha and 3T3 cell lines. Mocha cells (A–D) and 3T3 cells (E–H) were transfected with ΔpMEP constructs containing cDNA encoding wild-type human CD63 (A, B, E, and F) or the GYEVI construct (C, D, G, and H). Stably transfected cell lines were treated with 3 μM CdCl₂ to induce protein expression and then double labeled, for indirect immunofluorescence localization, with a mouse mAb to CD63 (A, C, E, and G) and a rat mAb to endogenous mouse LAMP-1 (B, D, F, and H). Bar, 20 μm.