Skip to main content
. 2023 Jan 30;10(2):54. doi: 10.3390/jcdd10020054

Figure 3.

Figure 3

Metformin inhibits local plaque and circulating MMP-9 activity in ApoE-/- mice. (a) Flowchart illustrating the experimental procedure for actuating metformin treatment in a collar-induced carotid atherosclerotic plaque model. (b) Representative images of immunofluorescence staining for active-matrix metalloproteinase (MMP)-9, MMP-2, and MMP-12 in plaque after metformin treatment. Scale bars represent 20 μm. (c) Quantification of immunofluorescence staining for MMP family in plaque after metformin treatment. Unpaired Student’s t-test, n = 6 per group. (d) A Gelatinase Assay Kit was used to detect relative MMP activity in serum. Unpaired Student’s t-test, n = 6 per group.