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. 2023 Jan 28;16(2):198. doi: 10.3390/ph16020198

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative indication of the change in (A) PLCζ fluorescence levels and (B) Ac + Eq/dispersed localisation ratios following AUM in non-optimal (dark bars) versus optimal (light bars) parameters of sperm motility (≥40%), sperm concentration (count; ≥15 × 10⁶ sperm/mL), and semen volume (1.5–5.5 mL). (C) PLCζ fluorescence levels and (D) Ac + Eq/dispersed localisation ratios following AUM in cases of unsuccessful fertilisation (dark bars) compared to cases of successful fertilisation (light bars). Asterisks (*, **, ***, ****) indicate a statistically significant (p ≤ 0.05) difference. Data are indicative of 100 cells examined from 55 patients each (550 cells). a.u.: arbitrary units.

Representative indication of the change in (A) PLCζ fluorescence levels and (B) Ac + Eq/dispersed localisation ratios following AUM in non-optimal (dark bars) versus optimal (light bars) parameters of sperm motility (≥40%), sperm concentration (count; ≥15 × 10⁶ sperm/mL), and semen volume (1.5–5.5 mL). (C) PLCζ fluorescence levels and (D) Ac + Eq/dispersed localisation ratios following AUM in cases of unsuccessful fertilisation (dark bars) compared to cases of successful fertilisation (light bars). Asterisks (*, **, ***, ****) indicate a statistically significant (p ≤ 0.05) difference. Data are indicative of 100 cells examined from 55 patients each (550 cells). a.u.: arbitrary units.