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AFM images of human spermatozoa fixed with different methods: (a) EtOH, (b) PFA 2%, and (c) PFA 3.7%. Scale bar is 10 µm. (d) Spermatozoa cell volume data expressed as a min-to-max box plot. A one-way ANOVA with multiple comparisons test was performed to test the statistical difference among the mean volumes obtained after fixations. ** p < 0.005 * p < 0.05.
