Figure 7.
AFM images with corresponding surface profiles collected on a selection of HEK-293 cells fixed with the different methods: (a) EtOH 70%, (b) PFA 2%, (c) PFA 3.7%, (d) 1:1 MeOH/C3H6O, and (e) cryofixed. Scale bar is 5 µm. Cell volume calculated from the AFM images collected on a selection of HEK-293 cells fixed with ethanol and PFA 3.7% and compared with the rest of the applied fixation methods (f). The second column shows the same cells depicted in Figure 6 (where a, b and e are rotated of 90 degrees left, 90 degrees right and 180 degrees respectively compared to Figure 6), while the other columns depict additional cells prepared with the same five fixation methods. * p < 0.05, ** p < 0.005, *** p < 0.001, **** p < 0.0005.