TABLE 3.
Phosphate release and accumulation of propionate in cell-free extracts of P. putida AC577(pLA82)a
| Substrate | Concn (mM) | Pi release ± SD | Propionate ± SDb |
|---|---|---|---|
| 2PP | 2 | 410 ± 12.5 | 400 ± 10.3 |
| 5 | 620 ± 21 | 560 ± 12.5 |
a
Pi release and propionate accumulation are given in nanomoles per milligram of protein measured in the samples after 12 h of incubation of cell-free extracts with 2PP at 30°C.
b
Control samples from which either 2PP or cell-free extract was omitted contained no measurable amounts of propionate after 12 h of incubation.