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. 2023 Feb 11;15(2):611. doi: 10.3390/pharmaceutics15020611

Figure 1.

Figure 1

Effects of the molar ratio of DOTAP in cationic-LP and peptide modification on the intracellular uptake of STR-CH2R4H2C-LP-siRNA lipoplexes. (a) ATTO-labeled liposome and (b) FAM-siRNA cellular uptake (%) in RPE cells. STR-CH2R4H2C-cationic-LP-siRNA lipoplexes with different molar ratios of DOTAP (0.125–0.750 µmol per 3 µmol of total lipid in cationic-LP) and STR-CH2R4H2C (0.0–2.0 mol%) were transfected into RPE cells (2.0 × 105 cells) in serum-free DMEM for 4 h (ATTO-DOPE 0.2 µM, FAM-siRNA 0.158 µM). The mean fluorescence intensity (MFI) was measured by flow cytometry at 10,000 events per cell. Each value represents the relative percent to each unmodified control lipoplex. Data represent the mean ± SD (n = 3). * p < 0.05, ** p < 0.01.