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Binary pump program used for fast constant flow elution with mobile phases A and B, as described in methods.
| Time (min) | A (%) | B (%) | Flow (mL/min) |
|---|---|---|---|
| 0.00 | 95 | 5 | 0.500 |
| 2.00 | 30 | 70 | 0.500 |
| 2.50 | 5 | 95 | 0.500 |
| 3.00 | 5 | 95 | 0.500 |
| 3.01 | 95 | 5 | 0.500 |
| 4.00 | 95 | 5 | 0.500 |
