Table 1.

Gradient elution program for the separation of phenolic compounds.

Steps of Elution (min)	Water-0.2% v/v Formic Acid	Acetonitrile-0.1% v/v Formic Acid	Flow Rate (μL/min)
0.0	90	10	300
0.5	80	20	300
4.0	70	30	300
4.1	50	50	350
4.5	50	50	350
5.1	35	65	350
7.0	0	100	350
8.0	0	100	350
9.0	0	100	300
9.1	90	10	300
15	90	10	300