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Effect of allicin on the expression of inflammatory cytokine genes in breast tissues. (A–D) mRNA expression levels of IL-1β, IL-6, IL-8, and TNF-α in breast tissues were detected using RT–qPCR with β-actin used as an endogenous control. (E–H) The protein expression levels of IL-6 and TNF-α in breast tissues were detected using Western blotting, and α-tubulin was used as a control. (I) Mammary gland MPO activity assay. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
