Figure 1.

Characterization of BSA-coated and uncoated polydopamine nanoparticles (PDNPs). (A) Evolution of the PDNP hydrodynamic diameter in the function of reaction time as measured by DLS. All samples were sonicated by tip sonication to remove potential agglomerates. (B) Normalized UV/Vis spectrum of DA (blue), BSA (red), 500 nm PDNPs (dashed blue) and 500 nm BSA-coated PDNPs (dashed red). (C) Size distributions representing the stability of uncoated 500 nm PDNPs in water (blue) and Opti-MEM (dashed blue), showing a clear size increase after incubation in Opti-MEM. BSA-coated PDNPs maintain the same size distribution in both waters (red) and Opti-MEM (dashed red). Measured by DLS for an incubation time of 15 min.