ML phylogenetic tree based on the 640 nt fragment of the HBV S-gene (nucleotide positions 157–796 by reference sequence NC_003977.2). Tree branches with a posterior probability of >90% are shown in red. Clusters containing sequences from this study and representing sub-genotypes D1, D2, D3, A2, and C2 are shown in separate panels. The GenBank database number, the HBV genotype, the country, and the year of isolation are indicated for each sequence. Sequences from this study are shown using the following colors and abbreviations: green (WT)—wild-type, red (IE)—immune-escape HBsAg variants with the exception of T118A/V, light blue (IE)—T118A/V variants, blue—reference sequences for HBV genotypes and sub-genotypes.