Figure 2.

Regulation of ferroportin levels in monocytes by secreted hepcidin activity. THP-1 cells were cultured in the absence (−Fe, control medium) or presence of P19 cell-conditioned medium, either non-stimulated or stimulated to secrete hepcidin. (A) FPN (upper panel) and GAPDH (lower panel) protein levels were determined from western blots. The green arrow indicates varying degrees of FPN degradation in representative samples. (B) Changes in FPN protein levels are expressed as the ratio of FPN to GAPDH. Data are normalized to the control medium treatment. Samples treated with stimulated medium showed an approximately 2-fold decrease in FPN relative to controls. Values are the mean ± SEM. *, p < 0.01.