Table 4.
The presence of biofilm-associated and virulence genes in enterococci isolated from raw milk and cheese samples.
| Genes | Milk Samples | Cheese Samples | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| E. faecalis (n = 42) | E. faecium (n = 5) | E. casseliflavus (n = 1) | E. gallinarum (n = 1) | Total (n = 49) |
E. faecalis (n = 29) | E. faecium (n = 5) | E. gallinarum (n = 2) | Total (n = 36) |
Total (n = 85) |
|
| gelE | 36 (85.7%) |
4 (80.0%) |
1 (100.0%) |
1 (100.0%) |
42 (85.7%) |
25 (86.2%) |
5 (100.0%) |
2 (100.0%) |
32 (88.9%) |
74 (87.1%) |
| esp | 13 (31.0%) |
2 (40.0%) |
0 (0.0%) |
0 (0.0%) |
15 (30.6%) |
8 (27.6%) |
4 (80.0%) |
1 (50.0%) |
13 (36.1%) |
28 (32.9%) |
| asa1 | 28 (66.7%) | 2 (40.0%) |
1 (100.0%) |
0 (0.0%) |
31 (63.3%) |
19 (65.5%) |
4 (80.0%) |
2 (100.0%) |
25 (69.4%) |
56 (65.9%) |
| cylA | 2 (4.8%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
2 (4.1%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
2 (2.4%) |
| agg | 12 (28.6%) |
2 (40.0%) |
0 (0.0%) |
0 (0.0%) |
14 (28.6%) |
13 (44.8%) |
0 (0.0%) |
1 (50.0%) |
14 (38.9%) |
28 (32.9%) |
| ebpA | 27 (64.3%) |
5 (100.0%) |
1 (100.0%) |
1 (100.0%) |
34 (69.4%) |
18 (62.1%) |
5 (100.0%) |
2 (100.0%) |
25 (69.4%) |
59 (69.4%) |
| ebpB | 25 (59.5%) |
3 (60.0%) |
1 (100.0%) |
1 (100.0%) |
30 (61.2%) |
21 (72.4%) |
5 (100.0%) |
1 (50.0%) |
27 (75.0%) |
57 (67.1%) |
| ebpC | 16 (38.1%) |
1 (20.0%) |
0 (0.0%) |
1 (100.0%) |
18 (36.7%) |
28 (96.6%) |
4 (80.0%) |
2 (100.0%) |
34 (94.4%) |
52 (61.2%) |
| pil | 17 (40.5%) |
1 (20.0%) |
0 (0.0%) |
1 (100.0%) |
19 (38.8%) |
26 (89.7%) | 3 (60.0%) |
2 (100.0%) |
31 (86.1%) |
50 (58.8%) |
| srt | 20 (47.6%) |
4 (80.0%) |
0 (0.0%) |
1 (100.0%) |
25 (51.0%) |
23 (79.3%) |
4 (80.0%) |
1 (50.0%) |
28 (77.8%) |
53 (62.4%) |
| fsrA | 0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
5 (17.2%) |
1 (20.0%) |
0 (0.0%) |
6 (16.7%) |
6 (7.1%) |
| fsrB | 1 (2.4%) |
2 (40.0%) |
0 (0.0%) |
0 (0.0%) |
3 (6.1%) |
7 (24.1%) |
1 (20.0%) |
0 (0.0%) |
8 (22.2%) |
11 (12.9%) |
| sprE | 1 (2.4%) |
0 (0.0%) |
0 (0.0%) |
0 (0.0%) |
1 (2.0%) |
6 (20.7%) |
0 (0.0%) |
0 (0.0%) |
6 (16.7%) |
7 (8.2%) |
gelE: gelatinase; esp: enterococcal surface protein, asa1: aggregation substance, cylA: cytolysin operon, agg: aggregation substance; ebpA, ebpB, epbC: endocarditis and biofilm-associated pili; pil: pili; srt: pilus-associated sortase; fsrA: response regulator; fsrB: signaling peptide; sprE: serine protease.