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. 2023 Feb 8;12(4):1356. doi: 10.3390/jcm12041356

Figure 3.

Figure 3

SGLT-2-dependent glucose transport in monocytes and endothelial cells. (A) CD14++CD16− monocytes were starved without glucose and serum for 2 h, along with either GLUT inhibitor or SGLT-2 inhibitor. Afterwards, the cells were exposed to the fluorescent-tagged derivative of glucose (2-NBDG) for 2–4 h in the presence of GLUT or SGLT-2 inhibitor or both in combination. Cells were then washed and analysed for intracellular fluorescence using FACS or fluorescence plate reader. n = 5. All data are means ± SEM. (B) HUVECs were starved without glucose and serum for 2 h, along with either GLUT inhibitor or SGLT-2 inhibitor. After that, the cells were exposed to the fluorescent-tagged derivative of glucose (2-NBDG) for 2–4 h in the presence of a GLUT or SGLT-2 inhibitor or both in combination. Cells were then washed and analysed for intracellular fluorescence using FACS or fluorescence plate reader. n = 5. All data are means ± SEM. (C) HCAECs were starved without glucose and serum for 2 h, along with either GLUT inhibitor or SGLT-2 inhibitor. Afterwards, the cells were exposed to the fluorescent-tagged derivative of glucose (2-NBDG) for 2–4 h in the presence of GLUT or SGLT-2 inhibitor or both in combination. Cells were then washed and analysed for intracellular fluorescence using FACS or fluorescence spectroscopy. n = 5. All data are means ± SEM. ns = non-significant. * p < 0.05, ** p < 0.01 and *** p < 0.001.