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. 2023 Feb 8;24(4):3417. doi: 10.3390/ijms24043417

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CRISPRi-mediated TMEM97 knockdown and its effect on cell viability. (A) qPCR analysis of TMEM97 knockdown using CRISPRi in ARPE19-KRAB. Results are presented as the mean ± SEM of three biological repeats, each with three technical repeats. ** indicates p < 0.01. (B) Cell viability analysis of ARPE19-KRAB following TMEM97 knockdown in the presence or absence of tBHP. Results are presented as the mean ± SEM of four biological repeats, each with four to six technical repeats. ns indicates not significant; * indicates p < 0.05.