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TMEM97 knockdown resulted in reduced ROS levels. Quantification of (A) total ROS levels using CellRox assay and (B) mitochondrial superoxide levels using MitoSox assay, following TMEM97 knockdown in ARPE19-KRAB in the presence or absence of tBHP. n = 6 repeats from 2 independent experiments, error bars represented SEM; ns indicates not significant; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.
