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vir gene expression in Walker A box ATP-binding site mutants. Shown are the results of an immunoblot analysis of VirB8 expression in strains induced with either 1 or 3 μM AS. A lanes, wild-type A348(pYW12); B lanes, AB20(pYW12); C lanes, AB20(pZL81); D lanes, AB20(pZL82); E lanes, AB20(pZL83); F lanes, AB20(pZL84).
