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. 2023 Feb 10;15(2):496. doi: 10.3390/v15020496

Figure 1.

Figure 1

SARS-CoV-2 replication in Calu-3 cells. SARS-CoV-2 nucleocapsid gene (N) expression was monitored upon SARS-CoV-2 infection in Calu-3 cells by RT-qPCR (A) using specific primers and Western blot (B) using anti-N (42kDa) and anti-Tubulin (55 kDa, control) antibodies. qPCR data were normalized to a reference gene (Actin beta, ACTB), reported to mock (unrelated negative control) and expressed using a relative quantitation method (ddCT). Statistical analysis. All data (A) presented were calculated from three biological replicate (n = 3) measurements ± SD. The ordinary two-way analysis of variance (ANOVA) and Šídák’s multiple comparisons test were used for statistical analysis. Statistically significant differences (fold change vs. mock) are indicated by stars (*): * p < 0.05; ** p < 0.01; **** p < 0.0001.