Figure 7.

Modulation of human ACE2 mRNA by hsa-miR-1246. Calu3 cells were co-transfected with Homo sapiens (hsa) human miR-1246 mimic (0, 25, 50 and 100 nM) and a psiCHECK2 reporter construct (50 ng; see Supplementary File S4), in which the Rluc reporter gene was coupled with the wild-type (WT) or mutated (MUT) human ACE2 3′ untranslated region (UTR). An unrelated, negative miRNA control (Mock) was used for normalization in addition to the internal normalizer Fluc. The concentration “0 nM” corresponds to the transfection-reagent-only control. Statistical analysis: Data were calculated from three biological replicates and expressed as means ± SD. The two-way analysis of variance (ANOVA) and Šídák’s multiple comparisons test were used. Statistically significant differences (fold change WT vs. MUT) are indicated as follows: * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, nonsignificant.