Fig. 3.

PKD1 activation loop autophosphorylation occurs in cis. (A) Activation loop autophosphorylation kinetics of PKD1^KD at low concentration. Error bars are the SD of three biologically independent experiments. (B) Activation loop autophosphorylation of kinase-dead PKD1^KD D706N in the presence and absence of excess wild-type PKD1^KD. Error bars are the SD of three biologically independent experiments. (C) Mass spectrometry analysis of the autophosphorylation reaction containing 10 nM wild-type PKD1^KD and 40 nM kinase-dead PKD1^KD D706N shown in panel (B) Phosphospecies of wild-type PKD1^KD are separated by 80 Da (double-headed arrows).