Fig. 4.

p53 hypomorphs share a predictive gene signature. (A) Classification scores for training set samples obtained through leave one out cross-validation based on total 143 unique genes. Probability of 0.5 is used as the classification threshold to calculate accuracy. (B) Classification scores for independent test set. (C) IPA analysis showing enrichment and effect on regulators with targets significantly enriched among the 143 genes from the classifier. (D) Western blot analysis of WT, R175C, and Y220H LCLs treated with 10 μM Nutlin-3a for 24 h. (E) Western blot analysis of WT, P47S, G360A, E11Q, and R110H LCLS treated with 10 μM Nutlin-3a for 24 h.