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Effect of ADSC secretome from ADSC cultivated in normoxia or hypoxia with or without EGF supplementation on keratinocyte migration and viability. (a) Migration of keratinocytes depicted as cell-free area over 24 h. (b) Keratinocyte viability measured by WST-8 over 7 days. Values were calculated relatively (24 h = 1). (n = 3, technical triplicates, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.005). (c) Representative images of keratinocyte migration after 0 h and 24 h. Scale bar 200 µm.
