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Glycerol kinase-dependent rescue of growth on lactose. Cells of strain AP110 that harbor plasmids with different glpK alleles were inoculated into minimal-lactose medium and incubated with shaking at 42°C. Growth was monitored by determining the OD600. ●, pCJ102 (normal glycerol kinase); ▪, pBR322 (vector); ▾, pCJ102-T477N (T-477-N glycerol kinase).
