FIG. 3.

Roles of glycerol kinase allosteric regulation in diauxic growth, glucose-glycerol utilization, and expression of glycerol kinase in the MC4100 genetic background. Cultures were prepared as described in Materials and Methods. (A) Filled symbols show growth of strains 34 (R⁺/Kⁱ) (▪), 24 (R⁺/K^r) (♦), and MC4100 (R⁺/K⁺) (●), which were measured as the change in OD₆₀₀ following inoculation. Open symbols connected by dashed lines show the concentration of glucose, and open symbols connected by solid lines show the concentration of glycerol in the medium. (B) Specific activity (S.A.) of glycerol kinase. ●, MC4100 (R⁺/K⁺); ▪, strain 34 (R⁺/Kⁱ); ♦, strain 24 (R⁺/K^r).