TABLE 1.
Strains and plasmids used in these studies
| Strain or plasmid | Genotype | Source or referencea |
|---|---|---|
| E. coli strain | ||
| DG1 | TB1 ΔglpK202 | 16 |
| HfrH | F+ | Ry Young |
| HfrPK3 | F+ayi-15 lacY1 leuB6 supE44 thi-1 thr-1 tonA21 | Ry Young |
| JM83 | F+ara glpR208 Δ(lac-proAB) rpsL(Strr) [φ80dlacΔ(lacZ)M15] thi | Ry Young; 17 |
| JW383 | thr-1 ara-14 eda-50 fhuA31 Δ(galK-attλ)99 hisG4(Oc) lacY1λ−leuB6 metF159(Am) mtl-1 rpsL136(Strr) thi-1 tsx-78 xylA5 zii-510::Tn10 | CGSC; 34 |
| Lin 43 | HfrC fadL701 glpK22 glpR2 phoA8 pit-10 relA1 sopT1 tonA22 T2r | CGSC; 42 |
| MC4100 | F−araD139 Δ(argF-lac)U169 deoC1 flbB5301 ptsF25 rbsR relA1 rpsL150(Strr) | CGSC |
| MG1655 | λ−rph-1 | CGSC |
| TB1 | JM83 glpR208 hsdR(rK− mK+) | 1 |
| ts1962 | F−lac-28 proA23 ptsI19(Ts) rpsL101(Strr) trp-30 | CGSC; 12 |
| AP100 | ptsI19(Ts) rpsL101(Strr) | ts1962 × HfrH |
| AP110 | AP100 ΔglpK202 zii-510::Tn10 | AP100 × P1.KH10 |
| KH1 | DG1 zii-510::Tn10 | DG1 × P1.JW383 |
| KH5 | HfrPK3 ΔglpK202 zii-510::Tn10 | Hfr PK3 × P1.KH1 |
| KH10 | KH1 pro+ | KH1 × HfrH |
| KH11 | DG1 glpK+pro+ | DG1 × Hfr PK3 |
| KH12 | KH10 glpK203 | KH10 × KH5/pA65T |
| KH15 | KH10 glpK204 | KH10 × KH5/pT477N |
| KH18 | MC4100 ΔglpK202 zii-510::Tn10 | MC4100 × P1.KH1 |
| KH24 | MC4100 glpK204 zii-510::Tn10 | MC4100 × P1.KH15 |
| KH34 | MC4100 glpK203 zii-510::Tn10 | MC4100 × P1.KH12 |
| KH37 | KH10 glpK22 | KH10 × KH5/pCH5 |
| KH38 | MC4100 glpK22 zii-510::Tn10 | MC4100 × P1.KH37 |
| KH51 | MG1655 ΔglpK202 zii-510::Tn10 | MG1655 × P1.KH1 |
| KH52 | MG1655 zii-510::Tn10 | MG1655 × P1.KH1 |
| KH58 | MG1655 glpK203 zii-510::Tn10 | MG1655 × P1.KH12 |
| KH59 | MG1655 glpK204 zii-510::Tn10 | MG1655 × P1.KH15 |
| KH61 | MG1655 glpK22 zii-510::Tn10 | MG1655 × P1.KH38 |
| Plasmids | ||
| pA65T | pHG165 + HindIII fragment glpK203 | 16 |
| pCH5 | pHG165 + HindIII fragment glpK22 | 21 |
| pCJ102 | pBR322 + HindIII fragment glpK | 23 |
| pCJ102/T477N | pBR322 + HindIII fragment glpK204 | This study |
| pHG165 | Cloning vector; ampr | 35 |
| pT477N | pHG165 + HindIII fragment glpK204 | This study |
a
CGSC, E. coli Genetic Stock Center, Yale University, New Haven, Conn.