Figure 4.

Nasal virus shedding following Dex-induced latency reactivation in PRV wt and PRVtmv+-infected pigs. Nasal swabs collected from pigs daily between 0 and 5 days post-dexamethasone injection (dp-Dex) were tested by qPCR targeting PRV–major capsid protein (MCP) gene. PRV genomic copy numbers were calculated according to the CT values of a standard curve. The mean copy numbers of PRV genome in 100 ng of total DNA from nasal swabs of two independent qPCR analyses of each animal from both groups on 0, 1, 2, 3, 4, and 5 dp-Dex injection. The values represent the mean for each animal in each group.