Table 2.

List of primers, probes, and double-stranded gene blocks (ds-gblock as standard) used in quantitative PCR for quantification of pseudorabies (PRV) ICP0, major capsid protein, and glycoprotein C gene/transcript copies in tissue samples and subsequent normalization based on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping gene.

Primer/Probe/ds-Gblock	Name	Sequence
PRV-ICP0	Forward	5′-atcccgtgctcctggataatctcg-3′
	Reverse	5′-tccccgtcttcaactggctttatg-3′
	Probe	5′Fam-atgttgtccacgacggcctcgcgga-3′ Tamra
	ds-gblock	5′-ggcctcggtcacgcgctggcggttcatcccgtgctcctggataatctcgacg Atgttgtccacgacggcctcgcggatggggtcgctctcgatgaccgtcgagacct gcccataaagccagttgaagacggggactctggggcgggcgcgagacccaga-3′
PRV–Major Capsid Protein	Forward	5′-ccatccagtttgaggtgcag-3′
	Reverse	5′-cgaggcgcttgatcatgtag-3′
	Probe	5′Fam-cccgtcgcgcgcgatcatcg-3′ Tamra
	ds-gblock	5′-ctcagctacgtggccgagggcaccatccagtttgaggtgcagcagccgatg atcgcgcgcgacgggccgcacccggccgaccagcccgtgcacaactacatgatca agc gcctcgatcgccgctccctcaacgccgc-3′
PRV–Glycoprotein C	Forward	5′-gtcgtccgcgactactacc-3′
	Reverse	5′-tcacgttcaccacggagac-3′
	Probe	5′Fam-cgtccgcgaaccagcgcagg-3′ Tamra
	ds-gblock	5′-agcccttccgggcggtgtgcgtcgtccgcgactactacccgcggcgcagcgtg cgcctgcgctggttcgcggacgagcacccggtggacgccgccttcgtgaccaa cagcaccgtggccgacgagctcgggcgccgcacgcgcgtctccgtggtgaac gtgacgcgcgcggacgtcccgggc-3′
Swine Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)	Forward	5′-atgacaacttcggcatcgtg-3′
	Reverse	5′-ccatccacagtcttctgggt-3′
	Probe	5′Fam-accacagtccatgccatcactgcc-3′ Tamra
	ds-gblock	5′-gcacccctggccaaggtcatccatgacaacttcggcatcgtggaaggactca tgaccacagtccatgccatcactgccacccagaagactgtggatggc ccctctgggaaacgtggcgt-3′