Fig. 4.

SspB contacts with ClpX RKH loops are poorly conserved. (A) The folded domain of SspB proximal to ClpX (green surface representation) is contacted by six RKH loops (sphere representation colored by subunit identity). (B) Conservation score of SspB residues calculated by Consurf (https://consurf.tau.ac.il/consurf_index.php) plotted against sequence position. Positive values indicate higher sequence conservation. Residues classified and bars colored based on SspB residue contacts. Gray bars (other) note residues lacking ClpX or SspB interdimer contacts. For this analysis, SspB residues within 5 Å from interacting molecules were considered as contacts.