FIG. 2.

seqA::MudI1734 and otsA mutations block the high-osmolality-dependent growth stimulation at 45°C. The growth rates of the wild-type strain TL1 (A), the seqA::MudI1734 mutant TL3283 (B), and the otsA::MudI1734 mutant TL3354 were determined at 45°C, as described in Materials and Methods. Symbols: ○, M63–20 mM glucose–0.5 mM methionine; ▵, medium supplemented with 0.2 M NaCl; ▫, medium supplemented with 0.2 M NaCl and 1 mM glycine betaine (GB); ⋄, medium supplemented with 10 mM galactose.