TABLE 1.
Growth rates of trehalose-deficient strains at different temperatures
| Temperature (°C) | Strain (genotype) | Growth ratea (generation/h ± SE) in medium plus:
|
||
|---|---|---|---|---|
| 0.0 M NaCl | 0.2 M NaCl | 0.2 M NaCl + GB | ||
| 37 | TL1 (wild type) | 1.31 ± 0.01 | 0.92 ± 0.01 | 1.07 ± 0.02 |
| TL3354 (otsA::MudI1734) | 1.18 ± 0.09 | 0.90 ± 0.01 | 1.12 ± 0.02 | |
| TL3283 (seqA::MudI1734) | 0.87 ± 0.07 | 0.77 ± 0.01 | 0.99 ± 0.01 | |
| 42 | TL1 (wild type) | 1.04 ± 0.08 | 0.88 ± 0.11 | 1.08 ± 0.03 |
| TL3354 (otsA::MudI1734) | 0.97 ± 0.02 | 0.67 ± 0.17 | 1.11 ± 0.02 | |
| TL3283 (seqA::MudI1734) | 0.68 ± 0.06 | 0.45 ± 0.03 | 0.82 ± 0.03 | |
| 45 | TL1 (wild type) | <0.03 | 0.40 ± 0.07 | 0.35 ± 0.05 |
| TL3354 (otsA::MudI1734) | <0.01 | <0.03 | 0.45 ± 0.04 | |
| TL3283 (seqA::MudI1734) | <0.02 | 0.02 | 0.21 ± 0.02 | |
a
The specific exponential growth rates of the strains were determined, as described in Materials and Methods, at the indicated temperatures in M63 medium–20 mm glucose–Met plus the indicated concentrations of NaCl or 1 mM glycine betaine (GB). The data are averages ± standard errors of results obtained in three independent experiments.