FIG. 2.

Northern blot analysis of the time course of fsrC and gelE expression. (A) Northern blot analysis during growth using an fsrC probe. (B) Northern blot analysis during growth using a gelE probe. Lanes: total RNA from E. faecalis OG1RF cells from 2-, 3-, 4-, 5-, 6-, 8-, and 12-h cultures. RNA isolation, Northern blotting, and hybridization were performed as described in Materials and Methods. (C) Growth curve of OG1RF. Bacterial cells were cultured as described in Materials and Methods. Cell concentrations (CFU per milliliter) were determined by serial dilutions and plating on BHI agar in triplicate.