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Northern blot analysis of fsrC expression at different cell densities. OG1RF cells from early exponential (2-h) and postexponential (4-h) phases were harvested by centrifugation and resuspended in BHI to desired concentrations and incubated at 37°C for 45 min before RNA was isolated for Northern blot analysis using the fsrC probe. (A) OG1RF cells from 2-h culture. (B) OG1RF cells from 4-h culture. The cell concentration (CFU per milliliter) used in each lane is indicated.
