FIG. 6.

Determination of the 5′ ends of the fsrA, fsrB, and gelE transcripts by primer extension. Primer extension was carried out as described in Materials and Methods. The primer extension products were run alongside sequencing reactions obtained with the same primers. Arrows, primer extension products in the sequencing gels; asterisks, locations of the 5′ ends of the sequences (left of each gel). The sequences encompassing the 5′ ends of the transcripts are shown on the top of each gel; putative −35, −10, and ribosome binding site (RBS) sequences are underlined, and the transcription start sites (+1) are in boldface and underlined. Primer extensions using primers APE1 (A), BPE2 (B), and EPE2 (C) are shown.