FIG. 9.

Schematic map of fsrB and gelE promoter regions in different lacZ fusion constructs and their β-galactosidase activities. (A) Different gelE promoter constructs, pTEX5270, pTEX5303, pTEX5304, pTEX5305, and pTEX5306, and their β-galactosidase activities in OG1RF. (B) Different fsrB promoter constructs, pTEX5269, pTEX5298, pTEX5299, pTEX5300, pTEX5301, and pTEX5302, and their β-galactosidase activities in OG1RF. The sequences for pTEX5270 and pTEX5269 (top lines of panels A and B) only show the regions containing the gelE and fsrB promoter and the repeated sequences; the sequences further upstream are not shown. All the gelE promoter constructs have the same sequences downstream of the promoter to bp −16 from gelE start codon (dots, sequence not shown), and all the fsrB promoter constructs have the same sequences downstream of the promoter to bp −8 from the fsrB start codon. The position labeled at the end of each line is relative to the gelE or fsrB start codon. Solid lines (A and B), sequences identical to that of the top sequences (changes in the promoter sequences are shown for constructs pTEX5305, pTEX5306, pTEX5301, and pTEX5302). Repeated sequences upstream of gelE and fsrB promoters are underlined; putative transcription start sites (+1) are in boldface and boxed; −10 and −35 sequences are also underlined. The β-galactosidase activity of each construct is indicated at the right.