Fig. 5.

Impact of palmitoylation on α1C association with β2a and trafficking. (A) Western blot showing the coimmunoprecipitation of WT and unpalmitoylatable α1C with β2a-CFP from HEK cells stably expressing WT and unpalmitoylatable α1C transfected with β2a-CFP. CL: detergent-solubilized cell lysate, IP: immunoprecipitated proteins. (B) Palmitoylation assay from HEK cells stably expressing WT and unpalmitoylatable α1C transfected with β2a-CFP (Left, +) or in the absence of β2a-CFP (Right, −). UF: unfractionated cell lysate, Palm: palmitoylated proteins. The bar chart shows α1C palmitoylation relative to expression in the presence (+) and absence (−) of β2a-CFP (N = 11). (C) Impact of α1C palmitoylation on its steady-state expression at the plasma membrane in the presence of β2a-CFP. Surface membrane proteins were biotinylated, purified, and immunoblotted for α1C and the housekeeping protein Na pump. The bar chart shows α1C surface membrane abundance relative to expression (N = 6).