FIG. 1.

Nucleotide sequence of C. nicotianae PDX2 gene. The nucleotides shown are sufficient to complement the two non-pdx1 pyridoxine auxotrophs, CS2 and CS7. The nucleotides in italics at either end represent the primers used to amplify this sequence from C. nicotianae genomic DNA. The underlined nucleotides are the primers used to amplify the RT-PCR product and to label the coding sequence with digoxigenin-11-dUTP. The putative AP-1 recognition sequence is shown in bold. The boxed nucleotides represent the regions of the CS2 and CS7 pdx2 genes that are missing a single nucleotide. The bold italic amino acid residues are identical in all known PDX2 proteins, while the additional amino acid residues shown in bold type are completely conserved only among fungal PDX2 proteins.