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. 2023 Jan 31;120(6):e2213765120. doi: 10.1073/pnas.2213765120

Fig. 4.

Fig. 4.

Summary of the effects of Middle NTR swaps in HSPB4 or HSPB5. (A) Installation of HSPB5 Middle NTR in HSPB4 increases HDX throughout the NTR at short exchange time points (4 s, 1 min, 30 min) and is associated with decreased secondary structure and increased chaperone activity (454-B4 vs. HSPB4). Installation of HSPB4 Middle NTR in HSPB5 decreases HDX throughout the NTR at short exchange time points (4 s, 1 min, 30 min) and is associated with increased secondary structure and decreased chaperone activity (B5-GFY or 545-B5 vs. HSPB5). (B) Summary of BPA cross-links identified from Middle subregion sites 24, 33, and 38 indicate the Critical sequence is a hub of NTR interactions.