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Western blot with L. lactis crude extracts prepared from glucose-grown wild-type and ptsH1 mutant strains and separated on a nondenaturing polyacrylamide gel. The various forms of HPr were detected with antibodies raised against B. subtilis HPr. Crude extracts from L. lactis wild-type MG5267 (lanes 1 and 2) and L. lactis ptsH1 mutant LlG101 (lanes 3 and 4) are shown. Extracts separated in lanes 2 and 4 were heated for 10 min at 65°C before they were loaded onto the gel.
