FIG. 4.

Transport of ¹⁴C-labeled TMG and mannitol and their exclusion by glucose in the L. lactis MG5267 (wild-type [wt]) and LlG101 (ptsH1 mutant) strains. (A) TMG transport with cells grown in M17 medium containing 0.5% lactose; (B) mannitol transport with cells grown in M17 medium containing 0.5% mannitol. Transport assays were carried out in the absence of glucose (squares) or with 10 mM glucose added 1 min prior to adding the radiolabeled sugar (circles). In panel B, the error bars for the experiments carried out in the presence of glucose were too small to be drawn by the program.