Fig. 3.

Cryo-EM structure determination of GRPR-G_q signaling complex. (A and B) Orthogonal views of the maps (Left) and models (Right) for the GRP (pale violet red) or [D-Phe⁶, β-Ala¹¹, Phe¹³, Nle¹⁴] Bn (6–14) (dark grey) bound GRPR-G_q complexes, with GRPR was colored in forest green and medium slate blue, respectively. Gα_q, Gβ, Gγ, and ScFv16 were colored in orange, orange red, yellow, and cyan, respectively. (C) Binding poses and sequence alignment of GRP and [D-Phe⁶, β-Ala¹¹, Phe¹³, Nle¹⁴] Bn (6–14). The density maps of ligands were highlighted.