Figure 4.

Ce6-mediated cytotoxicity on the melanoma cancer cells by the MTT assay. (A) Cytotoxic activity of Ce6 against B16F10 cells in the dark compared with vehicle-treated control. (B) Cytotoxic activity of Ce6-PDT against B16F10 cells compared with vehicle-treated control. (C) Morphological changes in B16F10 cells with or without Ce6 (192 µM, 384 µM, and 768 µM) in the dark and with or without Ce6 (6.25 µM, 12.5 µM, and 25 µM) followed by 660 nm laser at 50 mW for 200 s (1 J/cm²) in phototoxicity. The data are the presentation of mean ± SEM obtained from three independent experiments.