Th1-related cytokine expression level changes after Ce6- PDT treatment in B16F10 melanoma mice tumor. Real-time PCR expression of (A) TNF-α, (B). IFN-γ, and (C) IL-2 level in the non-irradiated right tumors and (D–F) in serum on the 4th and 7th day after Ce6-PDT. (G) Protein expression of TNF-α, IFN-γ, and IL-2 levels in the non-irradiated right tumors on the 4th and 7th day after Ce6-PDT treatment. Values are shown as mean ± SEM obtained from at least three independent experiments and were analyzed by one-way ANOVA with Tukey’s post hoc test. * p < 0.05 compared with the control group in 4 days. # p < 0.05, compared with the control group in 7 days.