Table 1.

Comparison of method 1 and method 2.

Parameters	Method 1	Method 2
The solvent used in the extraction	99.9 % EtOH	96% EtOH, hexane
Extraction time duration of chlorophyll a	12 h	12 h
Solvent/acid strength/time for demetallation (preparation of pheophytin a)	Hexane, 1 N HCl, 4 h	Hexane, 2 N HCl, 2 h
Base used for Ce6 synthesis	1 M NaOH	13% ethanolic solution of KOH
Time duration for Ce6 synthesis	12 h	10 min
Ce6 purification	pH change, centrifugation, and filtration	pH change, filtration
Total time	5 days	3 days
Yield % and Purity %	<1% of Spirulina mass and <97%	<1% of Spirulina mass and <94%