Figure 3.

Alterations in fibroblast growth factor receptor 1 (Fgfr1) methylation after alcohol consumption. (a) Schematic representation of significant changes in the methylation pattern of the murine Fgfr1 promoter. Chronic alcohol consumption led to hypermethylation of CpG −361 in dorsolateral striatum (DLS) and CpG −270 in prefrontal cortex (PFC). We used bisulfite conversion followed by direct capillary sequencing and two-tailed t-tests to identify the significantly mapped different CpG positions (total = 2). Arrowheads indicate significant alterations (p ≤ 0.05) in methylation rate (black = hypermethylation in the alcohol group compared with water-drinking control mice). (b) Estimated marginal means (EMM) of the methylation rate of all seven examined CpG sites in the Fgfr1 promoter in PFC and DLS of male mice after chronic alcohol exposure. Data are represented as mean ± SEM (n = 10 biological replicates) with a two-tailed Student’s t-test, * p ≤ 0.05; ** p ≤ 0.01 compared with the control group.