Fig. 3.

Reduced axon innervation of sympathetic targets in TrkA^R685A mice. (A–I) TH immunostaining of salivary glands (A–C), the olfactory epithelium (D–F), and the eye (G–I) reveals diminished sympathetic axon innervation in targets in E16 TrkA^R685A mice compared to littermate controls. (J–L) Further depletion of innervation in salivary glands by birth in TrkA^R685A mice revealed by TH immunostaining. Nuclei are stained with DAPI (blue). (Scale bar, 100 µm.) (C, F, I, and L) Quantification of TH-positive sympathetic nerves by measuring integrated fluorescence densities per unit area using ImageJ. Results are mean ± SEM from three to four mice per genotype and represented as a % of TrkA^WT values. *P < 0.05, **P < 0.01, one-sample t test with hypothetical mean of 100%.