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. 2023 Feb 1;120(6):e2212864120. doi: 10.1073/pnas.2212864120

Fig. 4.

Fig. 4.

circ_001400 inhibits viral gene expression and virion production. (A) Viral transcriptome analysis after circ_0001400 ectopic expression in KSHV-infected HUVECs (3 d post-infection). n = 3. Transcripts Per Kilobase Million were calculated and only KSHV genes with significant read counts are shown. (B) Viral transcript levels after circ_0001400 ectopic expression in KSHV-infected HUVECs. MOI of 0.5 (low) and 1.0 (high) i.u. are used, and RNAs were collected 3 d post-infection (n = 3). (C) Populations of GFP-positive, KSHV infected among lentivirus-infected HUVECs are shown. Cells were co-infected with KSHV and lentivirus (circGFP control or circ_0001400) for 3 d (n = 3). GFP signal from circGFP is significantly lower than KSHV BAC16-originated GFP and does not affect the analysis. (D) Viral transcript levels after circ_0001400 ectopic expression in KSHV-infected LECs. MOI of 1 i.u. was used for infection, and total RNAs were collected 3 d post-infection (n = 3). (E) Virus production from circ_0001400-depleted KSHV-infected LECs. Conditioned media of KSHV-infected LECs (D) was collected and used for infection of HEK-293 cells. KSHV-positive, GFP-positive population among HEK-293 cells were measured 3 d post-infection (n = 3). Significances were calculated with paired-t tests. *P-value < 0.05.