Fig. 6.

circ_001400 activates immune gene expression and increases co-stimulatory molecules on cell surface. (A) Human transcript levels after circ_0001400 ectopic expression in KSHV-infected HUVECs (MOI 0.5 i.u.). Total RNAs were extracted 3 d post-infection for RT-qPCR (n = 3). Significances were calculated with paired-t tests. *P-value < 0.05. (B and C) Immune staining of co-stimulatory molecules on circ_0001400-depleted KSHV-infected cells. At three dpi, cells were stained with antibodies specific to co-stimulators and isotypes (n = 3). Representative histograms are shown (B). Mean fluorescence intensity for each antibody was scaled to the signal from isotypes and shown in (C). Significances were calculated with paired-t tests. *P-value < 0.05.