Table 1.
Primer nucleotide sequences and annealing temperatures used for RT-PCR.
| Gene | Primer Nucleotide Sequences | T °C |
|---|---|---|
| Vinculin (VCL) | F: 5′-CTGAACCAGGCCAAAGGTT-3′ R: 5′-GATCTGTCTGATGGCCTGCT-3′ |
62 °C |
| Fibronectin (FN) | F: 5′-GAACTATGATGCCGACCAG-3′ R: 5′-GGTTGTGCAGATTTCCTCGT-3′ |
62 °C |
| Collagen type I-alpha chain 1 (Col1a1) | F: 5′-CGAGAGAGGTGAACAAGGC-3′ R: 5′-CCAGCATCACCCTTAGCACC-3′ |
55 °C |
| Integrin β-1 (ITGB1) | F: 5′-TGGAGGAAATGGTGTTTGC-3′ R: 5′- CGTTGCTGGCTTCACAAGTA-3′ |
55 °C |
| β-actin | F: 5′-ACTCTTCCAGCCTTCCTTCC-3′ R: 5′-GAGCCGCCGATCCACACG-3′ |
55 °C |